Journal: Journal of Diabetes Investigation

Article Title: Growth arrest‐specific 6 modulates adiponectin expression and insulin resistance in adipose tissue

doi: 10.1111/jdi.13412

Figure Lengend Snippet: Primer used for quantitative polymerase chain reaction analysis

Article Snippet: Thereafter, the tissue slides were then incubated at 4°C overnight with the following primary antibodies: Gas6 (G307; Bioworld) and adiponectin (AHP1005; AbD Serotec, Hercules, CA, USA).

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Journal of Diabetes Investigation

Article Title: Growth arrest‐specific 6 modulates adiponectin expression and insulin resistance in adipose tissue

doi: 10.1111/jdi.13412

Figure Lengend Snippet: Growth arrest‐specific 6 (Gas6) modulated the expression of adiponectin in adipocytes. (a) The efficiency of Gas6 knockdown in day‐7 (D7) adipocytes. (b) The adipocytokines gene expression (leptin, adiponectin, resistin and retinol‐binding protein 4 [RBP4]) after knockdown Gas6 in D7 adipocytes by real‐time polymerase chain reaction. (c) The adiponectin expression after knockdown Gas6 in D7 adipocytes by enzyme‐linked immunosorbent assay. Data are presented as the mean ± standard deviation. * P < 0.05. GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; mRNA, messenger ribonucleic acid.

Article Snippet: Thereafter, the tissue slides were then incubated at 4°C overnight with the following primary antibodies: Gas6 (G307; Bioworld) and adiponectin (AHP1005; AbD Serotec, Hercules, CA, USA).

Techniques: Expressing, Knockdown, Gene Expression, Binding Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Journal of Diabetes Investigation

Article Title: Growth arrest‐specific 6 modulates adiponectin expression and insulin resistance in adipose tissue

doi: 10.1111/jdi.13412

Figure Lengend Snippet: The expression of growth arrest‐specific 6 (Gas6) and adiponectin in insulin‐resistant obese mice. Mice fed a normal diet (ND; 10% of calories from fat) or high‐fat diet (HF; 60% of calories from fat) for 16 weeks, and blood samples were collected for measurement of blood glucose and insulin. (a) Bodyweight, fasting blood glucose, homeostasis model of assessment of insulin resistance and triglycerides levels are shown. (b) Immunohistochemistry images of Gas6 and adiponectin in adipocyte tissue (magnification: ×40). Data are presented as the mean ± standard deviation. * P < 0.05; n = 10 animals per group.

Article Snippet: Thereafter, the tissue slides were then incubated at 4°C overnight with the following primary antibodies: Gas6 (G307; Bioworld) and adiponectin (AHP1005; AbD Serotec, Hercules, CA, USA).

Techniques: Expressing, Immunohistochemistry, Standard Deviation

Journal: Journal of Diabetes Investigation

Article Title: Growth arrest‐specific 6 modulates adiponectin expression and insulin resistance in adipose tissue

doi: 10.1111/jdi.13412

Figure Lengend Snippet: Metformin ameliorated insulin resistance by enhancing the expression of growth arrest‐specific 6 (Gas6). Using thiazolidinedione (TZD) treatment day‐16 (D16) adipocytes treated with metformin (2 mmol/L) for 6 h. (a) The protein of Gas6, phospho‐adenosine monophosphate‐activated protein kinase (p‐AMPK), adenosine monophosphate‐activated protein kinase (AMPK), phospho‐insulin receptor substrate‐1 (p‐IRS1[307]), insulin receptor substrate‐1(IRS1), phospho‐protein kinase B (p‐Akt) and protein kinase B (Akt) expression by western blot. (b) The adipocytokine gene expressions (adiponectin, leptin, resistin and retinol‐binding protein 4 [RBP4]) by real‐time polymerase chain reaction. (c) The adiponectin expression after metformin treatment in day‐16 (D16) adipocyte by enzyme‐linked immunosorbent assay. Data were presented as the mean ± standard deviation. * P < 0.05. GAPDH, glyceraldehyde 3‐phosphate dehydrogenase.

Article Snippet: Thereafter, the tissue slides were then incubated at 4°C overnight with the following primary antibodies: Gas6 (G307; Bioworld) and adiponectin (AHP1005; AbD Serotec, Hercules, CA, USA).

Techniques: Expressing, Western Blot, Binding Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Drug Design, Development and Therapy

Article Title: 5-( Bis (3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors

doi: 10.2147/DDDT.S93570

Figure Lengend Snippet: Docking investigation of BHIMHA and staurosporine toward PKCα (PDB: 3IW4). Notes: ( A ) The feature of BHIMHA in the active site of PKCα, and the amplified interactions of energy minimized BHIMHA with the amino acid residues. ( B ) The feature of staurosporine in the active site of PKCα, and the amplified interactions of energy minimized staurosporine with the amino acid residues. Abbreviations: PKC, protein kinase C; BHIMHA, 5-( bis (3-(2-hydroxyethyl)-1H-indol-2-yl)-methyl)-2-hydroxybenzoic acid; staurosporine, (5S,6R,7R,9R)-6-methoxy-5-methyl-7-methylamino-6,7,8,9,15,16-hexahydro-5H,14H-17-oxa-4b,9a,15-triaza-5,9-methanodibenzo[ b, h ]cyclone-na[ jkl ]cyclopenta[ e ]asindacen-14-one; PDB, Protein Data Bank.

Article Snippet: PKCα, PKCβII, PKCγ, PKCη, and PKCδ antibodies were purchased from Boster Biological Engineering Co., Ltd. (Wuhan, People’s Republic of China); PKCθ antibody was purchased from CST (Shanghai) Biological Reagents Company Limited, Shanghai, People’s Republic of China.

Techniques: Amplification

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 1 Serum adiponectin concentrations in rabbits. 1, Aorta non-injured rabbits on normal cholesterol diet; 2, aorta injured rabbits on high-cholesterol diet before adenovirus transfer; 3, after transfer Ad-bgal through intima; 4, after transfer Ad-APN through intima; 5, after transfer Ad-bgal through adventitia; 6, After transfer Ad-APN through adventitia. The symbol * indicates significant difference (P!0.01) vs serum adiponectin concentrations in aorta non-injured rabbits on normal cholesterol diet; † compared with rabbits before adenovirus transfer; and ‡ compared with rabbits after Ad-bgal transfer. The values are meansGS.E.M.

Article Snippet: For immunohistochemical analyses, paraffin-imbedded cross-sections (5 mm thick) were incubated with either rabbit adiponectin polyclonal antibody (Boster), mouse vascular adhesion molecule-1 (VCAM-1) monoclonal antibody (Santa Cruz), mouse intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (Santa Cruz), mouse collagen I (Abcam, Cambridge, MA, USA), or mouse collagen III (Calbiochem, Darmstadt, Germany) monoclonal antibody.

Techniques:

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 2 SDS-PAGE (A) and western blot analysis of the supernatant of cultured Pichia pastoris (B, using anti-His tag monoclonal antibody; C, using adiponectin polyclonal antibody). Line 1 is a blank pGAPZa Pichia vector (not containing adiponectin gene) sample control and lines 2–4 are samples from the supernatant of cultured Pichia pastoris containing the recombinant rabbit adiponectin gene. Line 5 contained the molecular weight markers (not visible in image of blot). Both anti-His antibody and adiponectin polyclonal antibody detected a single predominant protein at z42 kDa (containing 9.3 kDa a-factor signal peptide and 2.5 kDa C-terminal His-tag).

Article Snippet: For immunohistochemical analyses, paraffin-imbedded cross-sections (5 mm thick) were incubated with either rabbit adiponectin polyclonal antibody (Boster), mouse vascular adhesion molecule-1 (VCAM-1) monoclonal antibody (Santa Cruz), mouse intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (Santa Cruz), mouse collagen I (Abcam, Cambridge, MA, USA), or mouse collagen III (Calbiochem, Darmstadt, Germany) monoclonal antibody.

Techniques: SDS Page, Western Blot, Cell Culture, Plasmid Preparation, Control, Recombinant, Molecular Weight

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 5 Effect of Ad-APN treatment on adhesion molecules in atherosclerotic rabbits 14 days after recombinant adenovirus transfer. A, Immunohistochemical detection of adiponectin, VCAM-1 and ICAM-1 in atherosclerotic lesions. Representative images are shown. 1, Ad-bgal transfer through intima. 2, Ad-APN transfer through intima. 3, Ad-bgal transfer through adventitia. 4, Ad-APN transfer through adventitia. The magnification is 400. B, mRNA expression of VCAM-1 and ICAM-1 in abdominal aortic tissue in Ad-bgal or Ad-APN-treated rabbits. The values are meansGS.E.M.

Article Snippet: For immunohistochemical analyses, paraffin-imbedded cross-sections (5 mm thick) were incubated with either rabbit adiponectin polyclonal antibody (Boster), mouse vascular adhesion molecule-1 (VCAM-1) monoclonal antibody (Santa Cruz), mouse intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (Santa Cruz), mouse collagen I (Abcam, Cambridge, MA, USA), or mouse collagen III (Calbiochem, Darmstadt, Germany) monoclonal antibody.

Techniques: Recombinant, Immunohistochemical staining, Expressing

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Autologous fat transplants to deliver glitazone and adiponectin for vasculoprotection

doi: 10.1016/j.jconrel.2017.08.036

Figure Lengend Snippet: Shown is the concentration of adiponectin detected in the media removed at day 4. N = 5 separate experiments using fat from five subjects. *P<0.05, 0 μM PGZ vs 60 μM PGZ; **P<0.01, 0 μM PGZ vs 600 μM PGZ.

Article Snippet: Nonspecific binding was blocked with 2% goat serum and tissue slices were incubated overnight at 4 °C with a rabbit anti-porcine adiponectin antibody (1:125 dilution) (Xeno Diagnostics, LLC).

Techniques: Concentration Assay

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Autologous fat transplants to deliver glitazone and adiponectin for vasculoprotection

doi: 10.1016/j.jconrel.2017.08.036

Figure Lengend Snippet: Fat with or without PGZ (200 μM) was placed perivascular to the left and right external jugular vein, respectively, in a pig. The veins were explanted at 2 weeks post-transplantation. The tissue was immunostained with anti-adiponectin antibody. Images A and B are from the vein exposed to the fat transplant with PGZ. Images C and D are from the vein exposed to the fat transplant without PGZ. Images A and B are of the vein wall and B and D are of the adipose tissue perivascular to the vessel wall. Dark gray indicates positive antibody binding. The length bar indicates 200 μm.

Article Snippet: Nonspecific binding was blocked with 2% goat serum and tissue slices were incubated overnight at 4 °C with a rabbit anti-porcine adiponectin antibody (1:125 dilution) (Xeno Diagnostics, LLC).

Techniques: Transplantation Assay, Binding Assay